AP7307a

antibody from Abcepta

Targeting: CA2 CA-II, CAII, Car2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7307a

Provider

Abcepta

Proper citation

Abgent Cat#AP7307a, RRID:AB_10613345

Product name

CA2 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

CA2 Antibody (N-term) (Cat.# AP7307a) western blot analysis in MCF-7 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the CA2 antibody detected the CA2 protein (arrow).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Fluorescent confocal image of Hela cell stained with CA2 Antibody (N-term)(Cat#AP7307a).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CA2 primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). CA2 immunoreactivity is localized to Cytoplasm significantly."

Primary Ab dilution

1:10~50