TA324917

antibody from OriGene

Targeting: CA2 CA-II, CAII, Car2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324917

Provider

OriGene

Product name

Rabbit polyclonal CA2 Antibody (N-term)

Antibody type

Polyclonal

Description

Rabbit polyclonal CA2 Antibody (N-term)

Host

Rabbit

Conjugate

Unconjugated

Epitope

CA2

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

2.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of lysates from MCF-7 cell line and mouse kidney tissue lysate (from left to right), using CA2 Antibody (N-term) (Cat. #TA324917). TA324917 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of Hela cell stained with CA2 Antibody (N-term)(Cat#TA324917).Hela cells were incubated with CA2 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . CA2 immunoreactivity is localized to Cytoplasm significantly.

Validation comment

IF