LS-C209667

antibody from LSBio

Targeting: CA2 CA-II, CAII, Car2

Western blot (Recommended by provider)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C209667

Provider

LSBio

Product name

CA2 / Carbonic Anhydrase II Antibody (Lyophilized, Biotin) LS-C209667

Antibody type

Polyclonal

Description

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Reactivity

Bovine

Host

Rabbit

Conjugate

Biotin

Isotype

IgG

Storage

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ELISA results of purified Rabbit Anti-CARBONIC ANHYDRASE II Biotin Conjugated Antibody tested against Carbonic Anhydrase II. Each well was coated in duplicate with 1.0 µg of Carbonic Anhydrase II. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-Streptavidin HRP conjugate, and TMB-1000 substrate.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA results of purified Rabbit Anti-CARBONIC ANHYDRASE II Biotin Conjugated Antibody tested against Carbonic Anhydrase II. Each well was coated in duplicate with 1.0 µg of Carbonic Anhydrase II. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-Streptavidin HRP conjugate, and TMB-1000 substrate.