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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA5-48006 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carbonic Anhydrase II Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Carbonic Anhydrase I, III, and XIII is observed and less than 1% cross-reactivity with rhCarbonic Anhydrase IV, VIII, IX, X, XII, and XIV is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Carbonic Anhydrase II in human heart tissue, human kidney tissue, Caki‚2 human clear cell carcinoma epithelial cell line, and A431 human epithelial carcinoma cell line. Samples were incubated in Carbonic Anhydrase II polyclonal antibody (Product # PA5-48006) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for Carbonic Anhydrase II/CA2 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Carbonic Anhydrase II in 0.2 mg/mL lysates of human kidney tissue, human heart tissue, and Caco‚2 human colorectal adenocarcinoma cell line. Samples were incubated in Carbonic Anhydrase II polyclonal antibody (Product # PA5-48006) using a dilution of 10 µg/mL followed by HRP-conjugated Anti-Sheep IgG at a dilution of 1:50. A specific band was detected for Carbonic Anhydrase II/CA2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-48006) and a ~30kDa band corresponding to CA2 was observed across cell lines and tissues except SW480, T-47D and Mouse Skeletal muscle which are reported to be negative. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), A-431 (Lane 2), SW480 (Lane 3), T-47D (Lane 4), tissue extracts (30ug lysate) of Mouse Colon (Lane 5), Mouse Kidney (Lane 6), Mouse Spleen (Lane 7) and Mouse skeletal muscle (Lane 8) were electrophoresed using NuPAGE® 10% Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (0.2ug/ml) and detected by chemiluminescence with Rabbit anti-Sheep IgG Secondary Antibody, HRP conjugate (product # 61-8620, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
