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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-51598 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carbonic Anhydrase II Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: YGKHNGPEHW HKDFPIAKGE RQSPVDIDTH TAKYDPSLKP LSVSYDQATS LRILNNGHAF NVEFDDSQDK AVLKGGPLDG TYRLIQFHFH WGSLDGQGSE HTVDKKKYAA ELHLVHWNTK YGDFGKAVQQ PDGLAVLG
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Carbonic Anhydrase II in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12 using a Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-51598).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Carbonic Anhydrase II in human cell lines HEK293 and PC-3 using a Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-51598). Corresponding CA2 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-51598) and a 29 kDa band corresponding to Carbonic Anhydrase II was observed across all the cell lines and tissues tested except PC-3, Hep G2 and mouse skeletal muscle (Fig. a and Fig. b). Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), A-431 (Lane 2), HEK-293 (Lane 3), PC-3 (Lane 4), Hep G2 (Lane 5), Mouse Stomach (Lane 6), Mouse Kidney (Lane 7), Rat Kidney (Lane 8), Mouse Skeletal Muscle (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.2 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Carbonic Anhydrase II was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-51598) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly cytoplasmic and plasma membrane localization. Panel e represents Hep G2 cells which has lower expression when compared to A-431 cells.. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Carbonic Anhydrase II in human stomach and prostate tissues using Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-51598). Corresponding CA2 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Carbonic Anhydrase II in human stomach using Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-51598) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Carbonic Anhydrase II in human prostate using Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-51598) shows low expression as expected.
