LS-C745100

antibody from LSBio

Targeting: CA2 CA-II, CAII, Car2

Western blot (Recommended by provider)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C745100

Provider

LSBio

Product name

CA2 / Carbonic Anhydrase II Antibody (Liquid, HRP) LS-C745100

Antibody type

Polyclonal

Description

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Reactivity

Bovine

Host

Rabbit

Conjugate

Horseradish Peroxidase

Isotype

IgG

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ELISA results of purified Rabbit Carbonic Anhydrase II Antibody tested against Carbonic Anhydrase II (erythrocytes). (Green line) The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA results of purified Rabbit Carbonic Anhydrase II Antibody tested against Carbonic Anhydrase II (erythrocytes). (Green line) The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.