Antibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [2]
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- Product number
- LS-C745133 - Provider product page
- Provider
- LSBio
- Product name
- CA2 / Carbonic Anhydrase II Antibody (Liquid, Biotin) LS-C745133
- Antibody type
- Polyclonal
- Description
- Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
- Reactivity
- Bovine
- Host
- Rabbit
- Conjugate
- Biotin
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ELISA results of purified Rabbit Anti-CARBONIC ANHYDRASE II Biotin Conjugated Antibody tested against Carbonic Anhydrase II. Each well was coated in duplicate with 1.0 µg of Carbonic Anhydrase II. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate, and TMB-1000 substrate.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA results of purified Rabbit Anti-CARBONIC ANHYDRASE II Biotin Conjugated Antibody tested against Carbonic Anhydrase II. Each well was coated in duplicate with 1.0 µg of Carbonic Anhydrase II. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate, and TMB-1000 substrate.