Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Flow cytometry [2]
- Other assay [2]
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- Product number
- PA5-78897 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carbonic Anhydrase II Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Zinc is a master-regulator of sperm function associated with binding, motility, and metabolic modulation during porcine sperm capacitation.
A Novel Sulfonamide, 4-FS, Reduces Ethanol Drinking and Physical Withdrawal Associated With Ethanol Dependence.
Zigo M, Kerns K, Sen S, Essien C, Oko R, Xu D, Sutovsky P
Communications biology 2022 Jun 3;5(1):538
Communications biology 2022 Jun 3;5(1):538
A Novel Sulfonamide, 4-FS, Reduces Ethanol Drinking and Physical Withdrawal Associated With Ethanol Dependence.
Sona Khan M, Trenet W, Xing N, Sibley B, Abbas M, Al-Rashida M, Rauf K, Mandyam CD
International journal of molecular sciences 2020 Jun 21;21(12)
International journal of molecular sciences 2020 Jun 21;21(12)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Carbonic Anhydrase II in Lane 1: rat ovary tissue lysate, Lane 2: mouse liver tissue lysate, Lane 3: MCF-7 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with Carbonic Anhydrase II polyclonal antibody (Product # PA5-78897) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CA2 in, Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse stomach tissue lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. The membrane was blocked with 5% non-fat milk/TBS for 1. 5 hour at RT. The membrane was incubated with Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-78897) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for CA2 at approximately 29 kDa. The expected band size for CA2 is at 29 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-78897) and ~30kDa band corresponding to CA2 was observed across cell lines and tissues except SW480 ,T-47D and Mouse Skeletal muscle along with an uncharacterized band (*) at ~48 kDa in the cell lines tested. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), A-431 (Lane 2), SW480 (Lane 3), T-47D (Lane 4), tissue extracts (30ug lysate) of Mouse Colon (Lane 5), Mouse Kidney (Lane 6), Mouse Spleen (Lane 7) and Mouse Skeletal muscle (Lane 8) were electrophoresed using NuPAGE® 10% Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (0.5ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Carbonic Anhydrase II on paraffin-embedded human gastric cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with Carbonic Anhydrase II polyclonal antibody (Product# PA5-78897) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Carbonic Anhydrase II on paraffin-embedded human liver cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with Carbonic Anhydrase II polyclonal antibody (Product# PA5-78897) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of Carbonic Anhydrase II in HT-29 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-78897) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of Carbonic Anhydrase II in SW480 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-78897) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Carbonic anhydrase type II (CA II) expression in the adult rat hippocampus. ( a ) Photomicrograph of CA II immunohistochemistry in the hippocampus and cortex from one control rat. CA II+ cells appeared as single cells; each immunoreactive cell is pointed with an arrowhead. 1- CA II+ cell in the hilus (Hil); 2-CA II+ cell in the molecular layer (Mol); 3-CA II+ cell in the corpus callosum (cc); 4-CA II+ cell in the cortex. ( b - i ) 100x images of the hilus used for quantitative analyses of CA II cells. ( e ) Zoomed in image shown in ( d ) to indicate the morphology of CA II+ cells in the hilus. Scale bar in ( e ) is 20 um; scale bar in ( i ) is 50 um (applies b - d and f - i ). ( j ) Number of CA II+ cells in the hilus. n = 5 controls, n = 5 ED, n = 4 CIE-ED, n = 3 vehicle controls, n = 3 4-FS controls, n = 8 4-FS ED rats, n = 7 4-FS CIE-ED rats. * p < 0.05, compared to controls; # p < 0.05 compared to 4-FS control. Data are expressed as mean +- S.E.M.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Phenotyping of three significantly different zincoproteins between non-capacitated and IVC spermatozoa. a - c CA2, d - e CCIN, and g - i CCDC39. IBFC of formaldehyde fixed and Triton X-100 permeabilized ( a , d ) or methanol fixed/permeabilized ( g ) spermatozoa; labeled with corresponding primary antibodies. Appropriate species-specific secondary antibodies conjugated to AF488 were used and coincubated with DAPI nuclear stain and PNA-AF647 for acrosome detection. Negative controls with respective non-immune sera of matching immunoglobulin concentration were performed and were published in our previous study. IBFC was performed in 5 replicates with consistent results. WB detection in individual fractions of zincoproteome purification ( b , e , h ), and WB detection in whole sperm extracts ( c , f , i ) were employed to fully characterize the capacitation changes in the studied proteins. The red arrows point to the bands of actual (CA2 ~ 32 kDa, CCIN ~ 60 kDa), and predicted (CCDC39 ~ 110 kDa) molecular weights.WB detection in sperm zincoproteome ( b , e , h ) and the whole sperm extracts ( c , f , i ) was performed in 8 replicates, and 3 replicates respectively. A statistically significant difference ( P < 0.05, two-sample t -test) between non-capacitated and capacitated spermatozoa is indicated by an asterisk.