Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-34925 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carbonic Anhydrase II Monoclonal Antibody (11A1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: THP-1, HL-60, rat liver tissue, human colon tissue, human kidney tissue, human stomach cancer tissue, mouse brain tissue.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 11A1
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Carbonic anhydrase 2 in Positive control: Lane 1: THP-1 cell lysate, untreated, Lane 2: HL-60 cell lysate, untreated. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with Carbonic anhydrase 2 monoclonal antibody (Product # MA5-34925), at a dilution of 1:1000, followed by Goat Anti-Mouse IgG - HRP (1 hour) with a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Carbonic anhydrase 2 in AGS cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Carbonic anhydrase 2 monoclonal antibody (Product # MA5-34925) at a dilution of 1:100 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Carbonic anhydrase 2 in 293T cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with Carbonic anhydrase 2 monoclonal antibody (Product # MA5-34925) at a dilution of 1:100 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Carbonic anhydrase 2 in paraffin-embedded rat liver tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Carbonic anhydrase 2 monoclonal antibody (Product # MA5-34925) at a dilution of 1:100 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Carbonic anhydrase 2 in paraffin-embedded mouse brain tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Carbonic anhydrase 2 monoclonal antibody (Product # MA5-34925) at a dilution of 1:100 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Carbonic anhydrase 2 in paraffin-embedded human stomach cancer tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Carbonic anhydrase 2 monoclonal antibody (Product # MA5-34925) at a dilution of 1:100 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Carbonic anhydrase 2 in paraffin-embedded human kidney tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Carbonic anhydrase 2 monoclonal antibody (Product # MA5-34925) at a dilution of 1:100 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Carbonic anhydrase 2 in paraffin-embedded human colon tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with Carbonic anhydrase 2 monoclonal antibody (Product # MA5-34925) at a dilution of 1:100 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
