PA5-28267

antibody from Invitrogen Antibodies

Targeting: CA2 CA-II, CAII, Car2

Antibody data

Submit

Validation data

Product number: PA5-28267 - Provider product page
Provider: Invitrogen Antibodies
Product name: Carbonic Anhydrase II Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: A431. Predicted reactivity: Dog (92%), Rabbit (89%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.17 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CA2 protein structure - PA5-28267 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Carbonic anhydrase 2 using 30 µg of A) 293T and B) A431 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Carbonic anhydrase 2 polyclonal antibody (Product # PA5-28267) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Carbonic Anhydrase II was performed by separating 30 µg of A431 lysates by 12% SDS PAGE. Proteins were transferred to a membrane and probed with a Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-28267) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Carbonic Anhydrase II was achieved by transfecting A-431 cells with Carbonic Anhydrase II specific siRNAs (Silencer® select Product # s2249; s2248). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the Carbonic Anhydrase II knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-28267, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Carbonic Anhydrase II.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of HEK- 293 (Lane 1), HeLa (Lane 2), HL-60 (Lane 3), HEL 92.1.7 (Lane 4), Caco-2 (Lane 5), A-431 (Lane 6), Caki-1 (lane 7), tissue extracts (30 µg lysate) of Mouse Spleen (Lane 8), Mouse Colon (Lane 9) and Mouse Kidney (Lane 10). The blot was probed with Anti-Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-28267, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 30 kDa band corresponding to Carbonic Anhydrase II was detected across the cell lines and tissues tested.