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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
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- Product number
- AF2184 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Carbonic Anhydrase II/CA2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Carbonic Anhydrase II/CA2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Carbonic Anhydrase I, III, and XIII is observed and less than 1% cross-reactivity with rhCarbonic Anhydrase IV, VIII, IX, X, XII, and XIV is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P00918- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Dissecting the Global Dynamic Molecular Profiles of Human Fetal Kidney Development by Single-Cell RNA Sequencing.
Wang P, Chen Y, Yong J, Cui Y, Wang R, Wen L, Qiao J, Tang F
Cell reports 2018 Sep 25;24(13):3554-3567.e3
Cell reports 2018 Sep 25;24(13):3554-3567.e3
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Carbonic Anhydrase II/CA2 by Western Blot. Western blot shows lysates of human heart tissue, human kidney tissue, Caki-2 human clear cell carcinoma epithelial cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Carbonic Anhydrase II/ CA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2184) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carbonic Anhydrase II/ CA2 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Carbonic Anhydrase II/CA2 by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue, human heart tissue, and Caco-2 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase II/CA2 at approximately 37 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Carbonic Anhydrase II/CA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2184) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
