MA1-24738

antibody from Invitrogen Antibodies

Targeting: DAPK1 DAPK, ROCO3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA1-24738

Provider

Invitrogen Antibodies

Product name

Phospho-DAPK1 (Ser308) Monoclonal Antibody (DKPS308)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

DKPS308

Vial size

50 µL

Concentration

2.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of Phospho-DAP Kinase 1 pSer308 in whole extract of HEK-293 cells overexpressing human DAP Kinase WT (Lane 1) and HEK-293 cells overexpressing human DAP-Kinase (mutant S308A). Samples were separated on SDS-PAGE and probed using a Phospho-DAP Kinase 1 pSer308 monoclonal antibody (Product # MA1-24738) at a dilution of 1 µg/mL and detected using a Goat anti-mouse IgG-Peroxidase and chemiluminescent substrate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Dapk1 Ser308ph was performed by loading whole cell lysate (in 1X SDS sample buffer with 2-ME) from 2 x 105 mouse embryonic fibroblasts. Samples were loaded onto a 4-12 % Bis-Tris polyacrylamide gel (Product # WG1402BOX). Proteins were transferred to nitrocellulose membrane by wet/tank transfer. Membrane was blocked in 5% milk/TBST and probed with a monoclonal anti-pDapk1 (S308) antibody (Product # MA1-24738) at a dilution of 1:1000 in 5% milk/TBST at 4°C overnight, followed by a secondary antibody HRP-anti-mouse at a dilution of 1:5000 at room temperature for 1 hour. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32209). The protein has a predicted MW of ~160 kDa.