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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- A300-220A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRD8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD8 by western blot of immunoprecipitates. Samples: Nuclear extract ( 0.5 or 1 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRD8 antibody A300-220A (lot A300-220A-2) used for IP at 6 µg per reaction. BRD8 was also immunoprecipitated by a previous lot of this antibody (lot A300-220A-1). For blotting immunoprecipitated BRD8, A300-220A-2 was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD8 by western blot. Samples: Nuclear extract (50 µg) from HeLa and 293T cells. Antibodies: Affinity purified rabbit anti-BRD8 antibody A300-220A (lot A300-220A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD8 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BRD8 (Cat. No. A300-220A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
