Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NB100-79781 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-79781, RRID:AB_1107487
- Product name
- Rabbit Polyclonal BRD8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: BRD8 Antibody [NB100-79781] - A) Nuclear extract (NE; 5, 15 and 50 mcg) from HeLa cells. B) Nuclear extract (1 mg) from HeLa cells. Antibodies: A) Rabbit anti-BRD8 antibody, NB100-79781 used at the indicated concentrations for WB. B). Rabbit anti-BRD8 antibodies, NB100-79780, and NB100-79781 used at 1 and 5 mcg/mg lysate for IP. Immunoprecipitated BRD8 was detected using anti-BRD8 antibody NB100-79781 at 0.1 mcg/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRD8 Antibody [NB100-79781] - Detection of Human BRD8 by Western Blot. Samples: Nuclear extract (50 ug) from HeLa and 293T cells. Antibodies: Affinity purified rabbit anti-BRD8 antibody NB100-79781 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BRD8 Antibody [NB100-79781] - Detection of human BRD8 by western blot of immunoprecipitates. Samples: Nuclear extract ( 0.5 or 1 mg per IP reaction; 20% of IP loaded ) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRD8 antibody NB100-79781 (lot NB100-79781-2) used for IP at 6 ug per reaction. BRD8 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79781-1). For blotting immunoprecipitated BRD8, NB100-79781 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: BRD8 Antibody [NB100-79781] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BRD8 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: BRD8 Antibody [NB100-79781] - FFPE section of human prostate adenocarcinoma. Affinity purified rabbit anti-BRD8 used at a dilution of 1:100.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BRD8 Antibody [NB100-79781] - Human prostate carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).