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Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- PA5-48625 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIST2H2AB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with rat, mouse, bovine and chicken based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Enrichment of endogenous H3.3 protein at specific gene loci using Anti-H3.3 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone H3.3 Monoclonal Antibody (Product # MA5-24667, 3 µg) on sheared chromatin from 2 million U-2 OS cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs for the GAPDH PROMOTER, GAPDH EXON2 used as positive and MYOD, SAT2 satellite repeats, SAT Alpha used as negative target genes/binding sites. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
