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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- R32566 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PC4 Antibody / Positive cofactor 4 / SUB1
- Antibody type
- Polyclonal
- Description
- This highly specific PC4 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PC4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat liver, 2) human HeLa and 3) human U-2 OS lysate with PC4 antibody at 0.5ug/ml. Expected molecular weight: 15-19 kDa (unmodified) and ~26 kDa (phosphorylated).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat liver, 2) rat spleen, 3) rat stomach, 4) rat RH35, 5) mouse liver and 6) mouse spleen tissue lysate with PC4 antibody. Expected molecular weight: 15-19 kDa (unmodified) and ~26 kDa (phosphorylated).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) A549, 3) MCF7, 4) T-47D, 5) PC-3, 6) Jurkat, 7) human placenta and 8) HL60 cell lysate with PC4 antibody. Expected molecular weight: 15-19 kDa (unmodified) and ~26 kDa (phosphorylated).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human intestinal cancer tissue with PC4 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 ETDA for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human breast cancer tissue with PC4 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse liver with PC4 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat liver with PC4 antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE type 1 human endometrioid adenocarcinoma tissue with PC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human esophageal squamous carcinoma tissue with PC4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
