MA5-29588

antibody from Invitrogen Antibodies

Targeting: SUB1 p14, p15, PC4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29588

Provider

Invitrogen Antibodies

Product name

PC4 Monoclonal Antibody (04)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human SUB1/PC4.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PC4 in Lane A: NIH-3T3 Whole Cell Lysate (30 µg), Lane B: Raji Whole Cell Lysate (30 µg) . Samples were probed using a PC4 Monoclonal Antibody (Product # MA5-29588) at a 1:500 dilution, followed by a Goat Anti-Mouse IgG (H+L), Dylight 800 Secondary Antibody at a 1:15000 dilution. Western blot was performed under reducing conditions. Predicted band size:14 kDa. Observed band size:16 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PC4 Monoclonal Antibody (4) (Product # MA5-29588) at 1:500 dilution. Lane A: NIH-3T3 Whole Cell Lysate, Lane B: Raji Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG H&L (DyLight™ 800) at 1:15,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 16 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Human PC4 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with PC4 Monoclonal Antibody (4) (Product # MA5-29588, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red) and counterstained with DAPI(blue). Positive staining was localized to nucleus.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PC4 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 µL with PC4 Monoclonal Antibody (4) (Product # MA5-29588) and 15 µL of 50 % Protein G agarose. Primary antibody: PC4 Monoclonal Antibody (4), at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7,500 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa.