PA5-117086

antibody from Invitrogen Antibodies

Targeting: SUB1 p14, p15, PC4

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117086

Provider

Invitrogen Antibodies

Product name

PC4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: HL-60, BT-474, SW620, Jurkat, Mouse brain Immunogen sequence: MPKSKELVSS SSSGSDSDSE VDKKLKRKKQ VAPEKPVKKQ KTGETSRALS SSKQSSSSRD DNMFQIGKMR YVSVRDFKGK VLIDIREYWM DPEGEMKPGR KGISLNPEQW SQLKEQISDI DDAVRKL

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.54 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PC4 in extracts of various cell lines. Samples were incubated with PC4 Polyclonal antibody (Product # PA5-117086) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PC4 in A549 cells. Samples were incubated with PC4 Polyclonal antibody (Product # PA5-117086).

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of PC4 in 150 μg extracts of HL60 cells. Samples were precipitated with 3 μg PC4 Polyclonal antibody (Product # PA5-117086). Western blot was performed from the immunoprecipitate using PC4 Polyclonal antibody (Product # PA5-117086) at a dilution of 1:500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of PC4 in 150 µg extracts of HL60 cells using PC4 Polyclonal Antibody (Product # PA5-117086). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:500