Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-48344 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRAMEF8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRAMEF8 in NCI-H460 cell line lysates (35 µg/lane). Lysates were probed with a PRAMEF8 Antibody (C-term) (Product # PA5-48344).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PRAMEF8 in formalin fixed and paraffin embedded human melanoma tissue. Samples were probed with a PRAMEF8 Antibody (C-term) (Product # PA5-48344) followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of PRAMEF8 in NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). Samples were incubated with a PRAMEF8 Antibody (C-term) (Product # PA5-48344) followed by incubation with a FITC-conjugated goat-anti-rabbit secondary antibody.