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- Antigen structure
- References [11]
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- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- 12105-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12105-1-AP, RRID:AB_2196111
- Product name
- SPIN1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SPIN1 antibody (Cat. #12105-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Epigenetic Control of Cancer Cell Proliferation and Cell Cycle Progression by HNRNPK via Promoting Exon 4 Inclusion of Histone Code Reader SPIN1.
UHRF1 is essential for proper cytoplasmic architecture and function of mouse oocytes and derived embryos.
Ubiquitin-independent proteasomal degradation of Spindlin-1 by the E3 ligase HACE1 contributes to cell-cell adhesion.
Structural mechanism of bivalent histone H3K4me3K9me3 recognition by the Spindlin1/C11orf84 complex in rRNA transcription activation.
The microRNA-381(miR-381)/Spindlin1(SPIN1) axis contributes to cell proliferation and invasion of colorectal cancer cells by regulating the Wnt/β-catenin pathway.
E2F1-activated SPIN1 promotes tumor growth via a MDM2-p21-E2F1 feedback loop in gastric cancer.
Soft Substrate Promotes Osteosarcoma Cell Self-Renewal, Differentiation, and Drug Resistance Through miR-29b and Its Target Protein Spin 1.
Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression.
SPIN1 promotes tumorigenesis by blocking the uL18 (universal large ribosomal subunit protein 18)-MDM2-p53 pathway in human cancer.
The Tudor domain protein Spindlin1 is involved in intrinsic antiviral defense against incoming hepatitis B Virus and herpes simplex virus type 1.
Nucleolar protein Spindlin1 recognizes H3K4 methylation and stimulates the expression of rRNA genes.
Li D, Guo J, Jia R
Journal of molecular biology 2023 Mar 15;435(6):167993
Journal of molecular biology 2023 Mar 15;435(6):167993
UHRF1 is essential for proper cytoplasmic architecture and function of mouse oocytes and derived embryos.
Uemura S, Maenohara S, Inoue K, Ogonuki N, Matoba S, Ogura A, Kurumizaka M, Yamagata K, Sharif J, Koseki H, Ueda K, Unoki M, Sasaki H
Life science alliance 2023 Aug;6(8)
Life science alliance 2023 Aug;6(8)
Ubiquitin-independent proteasomal degradation of Spindlin-1 by the E3 ligase HACE1 contributes to cell-cell adhesion.
Palicharla VR, Gupta D, Bhattacharya D, Maddika S
FEBS letters 2021 Feb;595(4):491-506
FEBS letters 2021 Feb;595(4):491-506
Structural mechanism of bivalent histone H3K4me3K9me3 recognition by the Spindlin1/C11orf84 complex in rRNA transcription activation.
Du Y, Yan Y, Xie S, Huang H, Wang X, Ng RK, Zhou MM, Qian C
Nature communications 2021 Feb 11;12(1):949
Nature communications 2021 Feb 11;12(1):949
The microRNA-381(miR-381)/Spindlin1(SPIN1) axis contributes to cell proliferation and invasion of colorectal cancer cells by regulating the Wnt/β-catenin pathway.
Zhou L, Wang H, Fang Z, Zhong M, He Y, Zou J, Huang S, Li J, Xiang X, Fang Z
Bioengineered 2021 Dec;12(2):12036-12048
Bioengineered 2021 Dec;12(2):12036-12048
E2F1-activated SPIN1 promotes tumor growth via a MDM2-p21-E2F1 feedback loop in gastric cancer.
Lv BB, Ma RR, Chen X, Zhang GH, Song L, Wang SX, Wang YW, Liu HT, Gao P
Molecular oncology 2020 Oct;14(10):2629-2645
Molecular oncology 2020 Oct;14(10):2629-2645
Soft Substrate Promotes Osteosarcoma Cell Self-Renewal, Differentiation, and Drug Resistance Through miR-29b and Its Target Protein Spin 1.
Li S, Bai H, Chen X, Gong S, Xiao J, Li D, Li L, Jiang Y, Li T, Qin X, Yang H, Wu C, You F, Liu Y
ACS biomaterials science & engineering 2020 Oct 12;6(10):5588-5598
ACS biomaterials science & engineering 2020 Oct 12;6(10):5588-5598
Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression.
Zhang X, Zhu G, Su X, Li H, Wu W
Biochemical and biophysical research communications 2018 Oct 20;505(1):325-332
Biochemical and biophysical research communications 2018 Oct 20;505(1):325-332
SPIN1 promotes tumorigenesis by blocking the uL18 (universal large ribosomal subunit protein 18)-MDM2-p53 pathway in human cancer.
Fang Z, Cao B, Liao JM, Deng J, Plummer KD, Liao P, Liu T, Zhang W, Zhang K, Li L, Margolin D, Zeng SX, Xiong J, Lu H
eLife 2018 Mar 16;7
eLife 2018 Mar 16;7
The Tudor domain protein Spindlin1 is involved in intrinsic antiviral defense against incoming hepatitis B Virus and herpes simplex virus type 1.
Ducroux A, Benhenda S, Rivière L, Semmes OJ, Benkirane M, Neuveut C
PLoS pathogens 2014 Sep;10(9):e1004343
PLoS pathogens 2014 Sep;10(9):e1004343
Nucleolar protein Spindlin1 recognizes H3K4 methylation and stimulates the expression of rRNA genes.
Wang W, Chen Z, Mao Z, Zhang H, Ding X, Chen S, Zhang X, Xu R, Zhu B
EMBO reports 2011 Oct 28;12(11):1160-6
EMBO reports 2011 Oct 28;12(11):1160-6
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12105-1-AP(SPIN1 antibody) at dilution of 1:800
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-SPIN1 (IP:12105-1-AP, 3ug; Detection:12105-1-AP 1:800) with HeLa cells lysate 1840ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The SPIN1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human SPIN1. This antibody recognizes human,mouse,rat antigen. The SPIN1 antibody has been validated for the following applications: ELISA, WB, IP, IHC analysis.
