Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C18752 - Provider product page
- Provider
- LSBio
- Product name
- RFX5 Antibody LS-C18752
- Antibody type
- Polyclonal
- Description
- Delipidation, salt fractionation and ion exchange chromatography
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 ug per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none.