Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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Comment
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- Product number
- H00005993-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005993-B01P, RRID:AB_10754848
- Product name
- RFX5 purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human RFX5 protein.
- Antigen sequence
MAEDEPDAKSPKTGGRAPPGGAEAGEPTTLLQRLR
GTISKAVQNKVEGILQDVQKFSDNDKLYLYLQLPS
GPTTGDKSSEPSTLSNEEYMYAYRWIRNHLEEHTD
TCLPKQSVYDAYRKYCESLACCRPLSTANFGKIIR
EIFPDIKARRLGGRGQSKYCYSGIRRKTLVSMPPL
PGLDLKGSESPEMGPEVTPAPRDELVEAACALTCD
WAERILKRSFSSIVEVARFLLQQHLISARSAHAHV
LKAMGLAEEDEHAPRERSSKPKNGLENPEGGAHKK
PERLAQPPKDLEARTGAGPLARGERKKSVVESSAP
GANNLQVNALVARLPLLLPRAPRSLIPPIPVSPPI
LAPRLSSGALKVATLPLSSRAGAPPAAVPIINMIL
PTVPALPGPGPGPGRAPPGGLTQPRGTENREVGIG
GDQGPHDKGVKRTAEVPVSEASGQAPPAKAAKQDI
EDTASDAKRKRGRPRKKSGGSGERNSTPLKSAAAM
ESAQSSRLPWETWGSGGEGNSAGGAERPGPMGEAE
KGAVLAQGQGDGTVSKGGRGPGSQHTKEAEDKIPL
VPSKVSVIKGSRSQKEAFPLAKGEVDTAPQGNKDL
KEHVLQSSLSQEHKDPKATPP- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of RFX5 expression in transfected 293T cell line (H00005993-T01) by RFX5 MaxPab polyclonal antibody.Lane 1: RFX5 transfected lysate(65.30 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to RFX5 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol