Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-118318 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TMEM92 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TMEM92 Polyclonal Antibody (Product # PA5-118318) at 1:500 dilution. Lane A: CACO-2 Whole Cell Lysate, Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 22 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TMEM92 Immunoprecipitation using: Lane A: 0.5 mg A549 Whole Cell Lysate 4 µL with TMEM92 Polyclonal Antibody (Product # PA5-118318) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: TMEM92 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 20 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TMEM92 Immunoprecipitation using: Lane A: 0.5 mg A549 Whole Cell Lysate 4 µL with TMEM92 Polyclonal Antibody (Product # PA5-118318) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: TMEM92 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 20 kDa.
