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antibody from Invitrogen Antibodies
Targeting: MIS18A
B28, C21orf45, C21orf46, FASP1, hMis18alpha
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-54238 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MIS18A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MAGVRSLRCS RGCAGGCECG DKGKCSDSSL LGKRLSEDSS RHQLLQKWAS MWSSMSEDAS VADMERAQLE EEAAAAEERP LVF
- Concentration
- 0.05 mg/mL
Submitted references Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery.
Qin W, Myers SA, Carey DK, Carr SA, Ting AY
Nature communications 2021 Aug 17;12(1):4980
Nature communications 2021 Aug 17;12(1):4980
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MIS18A in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Human cell line NTERA-2. Samples were probed using a MIS18A Polyclonal Antibody (Product # PA5-54238).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MIS18A in human cell line U-2 OS shows positivity in nucleus & cytoplasm. Samples were probed using a MIS18A Polyclonal Antibody (Product # PA5-54238).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MIS18A in human testis tissue shows strong nuclear positivity in cells in seminiferus ducts. Samples were probed using a MIS18A Polyclonal Antibody (Product # PA5-54238).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MIS18A in human testis and pancreas tissues using MIS18A Polyclonal Antibody (Product # PA5-54238). Corresponding MIS18A RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MIS18A in human pancreas using MIS18A Polyclonal Antibody (Product # PA5-54238) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Proteomic profiling of nucleolar RBPs by APEX-PS. a Numbers of proteins remaining after each step of filtering the mass spectrometric data using the ""pairwise ROC strategy"". The final nucleolar RBPome has 252 proteins (Supplementary Data 3 ). b Sample histogram showing how the cutoff for 130 C/126 C TMT ratio was applied. c GO cellular component analysis of nucleolar RBPs identified by APEX-PS. d GO biological process analysis of nucleolar RBPs identified by APEX-PS. e Molecular complexes enriched in nucleolar RBPome. Gray lines indicate protein-protein interactions annotated by the STRING database. f Nuclear specificity of nucleolar RBPome. g RBP specificity of nucleolar dataset. See Supplementary Data 3 for details. h Overlap with global RBP dataset obtained by OOPS . Bottom: Comparison of protein abundance for RBPs identified by both methods to those identified by APEX-PS only. Box limits represent 25th percentiles, medians, and 75th percentiles. Statistical analysis was performed with one-sided Wilcoxon rank sum test. i Validation of MIS18A and ATAD5 as novel nucleolar RBPs by UV crosslinking APEX-PS. j Validation of the RNA binding of MIS18A and ATAD5 by metabolic labeling of RNA by 5-EU and UV crosslinking. k Confocal imaging of endogenous MIS18A and ATAD5. Anti-FBL stains nucleolus and DAPI stains nuclei. Scale bars, 10 mum.
