PA5-54238

antibody from Invitrogen Antibodies

Targeting: MIS18A B28, C21orf45, C21orf46, FASP1, hMis18alpha

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-54238

Provider

Invitrogen Antibodies

Product name

MIS18A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MAGVRSLRCS RGCAGGCECG DKGKCSDSSL LGKRLSEDSS RHQLLQKWAS MWSSMSEDAS VADMERAQLE EEAAAAEERP LVF Highest antigen sequence identity to the following orthologs: Mouse - 41%, Rat - 57%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.05 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery.

Qin W, Myers SA, Carey DK, Carr SA, Ting AY
Nature communications 2021 Aug 17;12(1):4980

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of MIS18A in human cell line U-2 OS shows positivity in nucleus & cytoplasm. Samples were probed using a MIS18A Polyclonal Antibody (Product # PA5-54238).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 Proteomic profiling of nucleolar RBPs by APEX-PS. a Numbers of proteins remaining after each step of filtering the mass spectrometric data using the ""pairwise ROC strategy"". The final nucleolar RBPome has 252 proteins (Supplementary Data 3 ). b Sample histogram showing how the cutoff for 130 C/126 C TMT ratio was applied. c GO cellular component analysis of nucleolar RBPs identified by APEX-PS. d GO biological process analysis of nucleolar RBPs identified by APEX-PS. e Molecular complexes enriched in nucleolar RBPome. Gray lines indicate protein-protein interactions annotated by the STRING database. f Nuclear specificity of nucleolar RBPome. g RBP specificity of nucleolar dataset. See Supplementary Data 3 for details. h Overlap with global RBP dataset obtained by OOPS . Bottom: Comparison of protein abundance for RBPs identified by both methods to those identified by APEX-PS only. Box limits represent 25th percentiles, medians, and 75th percentiles. Statistical analysis was performed with one-sided Wilcoxon rank sum test. i Validation of MIS18A and ATAD5 as novel nucleolar RBPs by UV crosslinking APEX-PS. j Validation of the RNA binding of MIS18A and ATAD5 by metabolic labeling of RNA by 5-EU and UV crosslinking. k Confocal imaging of endogenous MIS18A and ATAD5. Anti-FBL stains nucleolus and DAPI stains nuclei. Scale bars, 10 mum.