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antibody from Invitrogen Antibodies
Targeting: MIS18A
B28, C21orf45, C21orf46, FASP1, hMis18alpha
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-53771 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MIS18A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KNLDYKRDLF CLSVEAIESY VLGSSEKQIV SEDKELFNLE SRVEIEKSLT QMEDVLKALQ MKLWEAESKL SFATC
- Concentration
- 0.20 mg/mL
Submitted references Mechanism of centromere recruitment of the CENP-A chaperone HJURP and its implications for centromere licensing.
Pan D, Walstein K, Take A, Bier D, Kaiser N, Musacchio A
Nature communications 2019 Sep 6;10(1):4046
Nature communications 2019 Sep 6;10(1):4046
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MIS18A in Lane 1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10; Lane 2: Human cell line NTERA-2. Samples were probed using a MIS18A Polyclonal Antibody (Product # PA5-53771).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MIS18A in human testis tissue shows strong nuclear positivity in cells in seminiferus ducts. Sertoli cells are generally negative. Samples were probed using a MIS18A Polyclonal Antibody (Product # PA5-53771).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MIS18A in human testis and pancreas tissues using MIS18A Polyclonal Antibody (Product # PA5-53771). Corresponding MIS18A RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MIS18A in human pancreas using MIS18A Polyclonal Antibody (Product # PA5-53771) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 8 Mis18 V211D is a separation-of-function mutant with impaired HJURP binding but not M18BP1 binding. a Mutated residues of Mis18alpha and Mis18beta are indicated on the 3D model. b , c SDS-PAGE results of the amylose-resin pull-down assays for testing the interaction of the mutants of Mis18alpha and Mis18beta with HJURP 394-C , M18BP1 1-60 , and M18BP1 61-140 . d Western blotting analysis confirming transgene expression. Endo. endogenous. e Representative images showing the fluorescence of mCherry-HJURP and EGFP-Mis18alpha variants in fixed HeLa cells treated as described in Fig. 2a but without the blocking and labeling steps of SNAP-CENP-A. Mis18alpha siRNA was used instead of HJURP siRNA. White scale bars indicate 10 um. f , g Quantification of the centromere fluorescence intensity of mCherry-HJURP and EGFP-Mis18alpha variants. The bar graphs represent mean values from three replicate experiments (blue dots indicate the mean values from each experiment). Error bars indicate standard deviations. h Two models of HJURP recruitment to the Mis18 receptor and the deposition of CENP-A:H4 dimers. Source data are provided as a Source Data file
