Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- V2934 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ZAP-70 Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant human protein (including amino acids 1-254 and the SH2 domains) was used as the immunogen for the ZAP-70 antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 2F3.2
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the ZAP-70 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Expression of TIA1 and PAX5 in Classical Hodgkin Lymphoma at Initial Diagnosis May Predict Clinical Outcome.
Prospective validation of predictive value of abdominal computed tomography scan on time to first treatment in Rai 0 chronic lymphocytic leukemia patients: results of the multicenter O-CLL1-GISL study.
Multiplexed Fluorescent Immunodetection Using Low Autofluorescence Immobilon®-FL Membrane.
Is ZAP70 still a key prognostic factor in early stage chronic lymphocytic leukaemia? Results of the analysis from a prospective multicentre observational study.
ROZA-XL, an improved FRET based biosensor with an increased dynamic range for visualizing zeta associated protein 70 kD (ZAP-70) tyrosine kinase activity in live T cells.
Serum thymidine kinase activity: analytical performance, age-related reference ranges and validation in chronic lymphocytic leukemia.
Apoptotic and proliferative characteristics of proliferation centers in lymph node sections of patients with chronic lymphocytic leukemia.
Prospective validation of a risk score based on biological markers for predicting progression free survival in Binet stage A chronic lymphocytic leukemia patients: results of the multicenter O-CLL1-GISL study.
microRNAome expression in chronic lymphocytic leukemia: comparison with normal B-cell subsets and correlations with prognostic and clinical parameters.
Nguyen TT, Frater JL, Klein J, Chen L, Bartlett NL, Foyil KV, Kreisel FH
Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry 2016 Jul;24(6):383-91
Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry 2016 Jul;24(6):383-91
Prospective validation of predictive value of abdominal computed tomography scan on time to first treatment in Rai 0 chronic lymphocytic leukemia patients: results of the multicenter O-CLL1-GISL study.
Gentile M, Cutrona G, Molica S, Ilariucci F, Mauro FR, Di Renzo N, Di Raimondo F, Vincelli I, Todoerti K, Matis S, Musolino C, Fabris S, Lionetti M, Levato L, Zupo S, Angrilli F, Consoli U, Festini G, Longo G, Cortelezzi A, Musto P, Federico M, Neri A, Ferrarini M, Morabito F
European journal of haematology 2016 Jan;96(1):36-45
European journal of haematology 2016 Jan;96(1):36-45
Multiplexed Fluorescent Immunodetection Using Low Autofluorescence Immobilon®-FL Membrane.
Park J, Mabuchi M, Sharma A
Methods in molecular biology (Clifton, N.J.) 2015;1314:195-205
Methods in molecular biology (Clifton, N.J.) 2015;1314:195-205
Is ZAP70 still a key prognostic factor in early stage chronic lymphocytic leukaemia? Results of the analysis from a prospective multicentre observational study.
Morabito F, Cutrona G, Gentile M, Fabris S, Matis S, Vigna E, Todoerti K, Colombo M, Recchia AG, Bossio S, De Stefano L, Ilariucci F, Cortelezzi A, Consoli U, Vincelli I, Pesce EA, Musolino C, Molica S, Di Raimondo F, Neri A, Ferrarini M
British journal of haematology 2015 Feb;168(3):455-9
British journal of haematology 2015 Feb;168(3):455-9
ROZA-XL, an improved FRET based biosensor with an increased dynamic range for visualizing zeta associated protein 70 kD (ZAP-70) tyrosine kinase activity in live T cells.
Cadra S, Gucciardi A, Valignat MP, Theodoly O, Vacaflores A, Houtman JC, Lellouch AC
Biochemical and biophysical research communications 2015 Apr 10;459(3):405-10
Biochemical and biophysical research communications 2015 Apr 10;459(3):405-10
Serum thymidine kinase activity: analytical performance, age-related reference ranges and validation in chronic lymphocytic leukemia.
Szánthó E, Bhattoa HP, Csobán M, Antal-Szalmás P, Újfalusi A, Kappelmayer J, Hevessy Z
PloS one 2014;9(3):e91647
PloS one 2014;9(3):e91647
Apoptotic and proliferative characteristics of proliferation centers in lymph node sections of patients with chronic lymphocytic leukemia.
Sachanas S, Levidou G, Angelopoulou MK, Moschogiannis M, Yiakoumis X, Kalpadakis C, Vassilakopoulos TP, Kontopidou F, Tsirkinidis P, Dimitrakopoulou A, Kokoris S, Dimitriadou E, Kyrtsonis MC, Panayiotidis P, Papadaki H, Patsouris E, Korkolopoulou P, Pangalis GA
Leukemia & lymphoma 2014 Mar;55(3):571-82
Leukemia & lymphoma 2014 Mar;55(3):571-82
Prospective validation of a risk score based on biological markers for predicting progression free survival in Binet stage A chronic lymphocytic leukemia patients: results of the multicenter O-CLL1-GISL study.
Gentile M, Cutrona G, Mosca L, Matis S, Fabris S, Lionetti M, Ilariucci F, Zupo S, Musolino C, Levato L, Molica S, Di Raimondo F, Vincelli I, Di Rienzo N, Pesce EA, Angrilli F, Federico M, Neri A, Ferrarini M, Morabito F
American journal of hematology 2014 Jul;89(7):743-50
American journal of hematology 2014 Jul;89(7):743-50
microRNAome expression in chronic lymphocytic leukemia: comparison with normal B-cell subsets and correlations with prognostic and clinical parameters.
Negrini M, Cutrona G, Bassi C, Fabris S, Zagatti B, Colombo M, Ferracin M, D'Abundo L, Saccenti E, Matis S, Lionetti M, Agnelli L, Gentile M, Recchia AG, Bossio S, Reverberi D, Rigolin G, Calin GA, Sabbioni S, Russo G, Tassone P, Morabito F, Ferrarini M, Neri A
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Aug 1;20(15):4141-53
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Aug 1;20(15):4141-53
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human Jurkat cell lysate with ZAP-70 antibody (clone 2F3.2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human tonsil stained with ZAP-70 antibody (2F3.2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free ZAP-70 Antibody (clone 2F3.2). Confirmation of Integrity and Purity of the Antibody.