HPA001356

antibody from Atlas Antibodies

Targeting: MYO5A GS1, MYH12, MYO5, MYR12

Western blot

Antibody data

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Validation data

Product number: HPA001356 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA001356, RRID:AB_1079441
Product name: Anti-MYO5A
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human MYO5A, Gene description: myosin VA (heavy chain 12, myoxin), Alternative Gene Names: GS1, MYH12, MYO5, MYR12, Validated applications: ICC, IHC, WB, Uniprot ID: Q9Y4I1, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.1 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

MYO5A protein structure - HPA001356 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human cerebral cortex and liver tissues using HPA001356 antibody. Corresponding MYO5A RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in pyramidal cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human kidney shows moderate membranous positivity in cells in tubules.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex shows strong membranous positivity in neuropil.
Sample type: HUMAN