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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- PA5-120601 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MT-ND3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive test controls include: 293T, PC-3, Mouse heart, Mouse brain. Immunogen sequence: MNFALILMIN TLLALLLMII TFWLPQLNGY MEKSTPYECG FDPMSPARVP FSMKFFLVAI TFLLFDLEIA
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.56 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MT-ND3 in L929 cells. Samples were incubated with MT-ND3 Polyclonal antibody (Product # PA5-120601) using a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MT-ND3 in HepG2 cells. Samples were incubated with MT-ND3 Polyclonal antibody (Product # PA5-120601) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of MT-ND3 in 200 μg extracts of HeLa cells. Samples were precipitated with 3 μg MT-ND3 Polyclonal antibody (Product # PA5-120601). Western blot was performed from the immunoprecipitate using MT-ND3 Polyclonal antibody (Product # PA5-120601) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MT-ND3 in paraffin-embedded human appendix. Samples were incubated with MT-ND3 Polyclonal antibody (Product # PA5-120601) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MT-ND3 in paraffin-embedded mouse kidney. Samples were incubated with MT-ND3 Polyclonal antibody (Product # PA5-120601) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MT-ND3 in paraffin-embedded human colon carcinoma. Samples were incubated with MT-ND3 Polyclonal antibody (Product # PA5-120601) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
