17817-1-AP

antibody from Proteintech Group

Targeting: RAB27A GS2, HsT18676, RAB27, RAM

Provider product page for 17817-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [55]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 17817-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#17817-1-AP, RRID:AB_2176728
Product name: RAB27A antibody
Antibody type: Polyclonal
Description: KD/KO validated RAB27A antibody (Cat. #17817-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IHC, IP, WB, ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

RAB27A protein structure - 17817-1-AP shown in red.

Submitted references Insulin granule morphology and crinosome formation in mice lacking the pancreatic β cell-specific phogrin (PTPRN2) gene.

Yasui T, Mashiko M, Obi A, Mori H, Ito-Murata M, Hayakawa H, Kikuchi S, Hosaka M, Kubota C, Torii S, Gomi H
Histochemistry and cell biology 2024 Mar;161(3):223-238

Activation of the Wnt/β-catenin signalling pathway enhances exosome production by hucMSCs and improves their capability to promote diabetic wound healing.

Wang L, Chen J, Song J, Xiang Y, Yang M, Xia L, Yang J, Hou X, Chen L, Wang L
Journal of nanobiotechnology 2024 Jun 26;22(1):373

Extracellular-vesicle-packaged S100A11 from osteosarcoma cells mediates lung premetastatic niche formation by recruiting gMDSCs.

Deng C, Xu Y, Chen H, Zhu X, Huang L, Chen Z, Xu H, Song G, Lu J, Huang W, Liu R, Tang Q, Wang J
Cell reports 2024 Feb 27;43(2):113751

Evoked Weibel-Palade Body Exocytosis Modifies the Endothelial Cell Surface by Releasing a Substrate-Selective Phosphodiesterase.

Naß J, Terglane J, Zeuschner D, Gerke V
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Apr;11(16):e2306624

KCa3.1 Promotes Proinflammatory Exosome Secretion by Activating AKT/Rab27a in Atrial Myocytes during Rapid Pacing.

Liu D, Chen H, Fu Y, Yao Y, He S, Wang Y, Cao Z, Wang X, Yang M, Zhao Q
Cardiovascular therapeutics 2023;2023:3939360

Development of a HiBiT-tagged reporter hepatitis E virus and its utility as an antiviral drug screening platform.

Nagashima S, Primadharsini PP, Nishiyama T, Takahashi M, Murata K, Okamoto H
Journal of virology 2023 Sep 28;97(9):e0050823

Selective pharmacological inhibition alters human carcinoma lung cell-derived extracellular vesicle formation.

Ipinmoroti AO, Pandit R, Crenshaw BJ, Sims B, Matthews QL
Heliyon 2023 Jun;9(6):e16655

Exosomal microRNA-4535 of Melanoma Stem Cells Promotes Metastasis by Inhibiting Autophagy Pathway.

Liu D, Li X, Zeng B, Zhao Q, Chen H, Zhang Y, Chen Y, Wang J, Xing HR
Stem cell reviews and reports 2023 Jan;19(1):155-169

Exosomal EIF5A derived from Lewis lung carcinoma induced adipocyte wasting in cancer cachexia.

Xiong H, Ye J, Luo Q, Li W, Xu N, Yang H
Cellular signalling 2023 Dec;112:110901

Pancreatic cancer-derived small extracellular vesical ezrin activates fibroblasts to exacerbate cancer metastasis through STAT3 and YAP-1 signaling pathways.

Chang YT, Peng HY, Hu CM, Tien SC, Chen YI, Jeng YM, Chang MC
Molecular oncology 2023 Aug;17(8):1628-1647

A Proximal-to-Distal Survey of Healthy Adult Human Small Intestine and Colon Epithelium by Single-Cell Transcriptomics.

Burclaff J, Bliton RJ, Breau KA, Ok MT, Gomez-Martinez I, Ranek JS, Bhatt AP, Purvis JE, Woosley JT, Magness ST
Cellular and molecular gastroenterology and hepatology 2022;13(5):1554-1589

High-Metastatic Melanoma Cells Promote the Metastatic Capability of Low-Metastatic Melanoma Cells via Exosomal Transfer of miR-411-5p.

Chen H, Zeng B, Li X, Zhao Q, Liu D, Chen Y, Zhang Y, Wang J, Xing HR
Frontiers in oncology 2022;12:895164

Melanoma stem cells promote metastasis via exosomal miR-1268a inactivation of autophagy.

Li X, Liu D, Chen H, Zeng B, Zhao Q, Zhang Y, Chen Y, Wang J, Xing HR
Biological research 2022 Oct 1;55(1):29

Novel RAB27A Variant Associated with Late-Onset Hemophagocytic Lymphohistiocytosis Alters Effector Protein Binding.

Zondag TCE, Torralba-Raga L, Van Laar JAM, Hermans MAW, Bouman A, Hollink IHIM, Van Hagen PM, Briggs DA, Hume AN, Bryceson YT
Journal of clinical immunology 2022 Nov;42(8):1685-1695

HBV induced the discharge of intrinsic antiviral miRNAs in HBV-replicating hepatocytes via extracellular vesicles to facilitate its replication.

Chu Q, Li J, Chen J, Yuan Z
The Journal of general virology 2022 May;103(5)

Acute Myeloid Leukemia Cells Educate Mesenchymal Stromal Cells toward an Adipogenic Differentiation Propensity with Leukemia Promotion Capabilities.

Zhang L, Zhao Q, Cang H, Wang Z, Hu X, Pan R, Yang Y, Chen Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Jun;9(16):2105811

Combination of Tipifarnib and Sunitinib Overcomes Renal Cell Carcinoma Resistance to Tyrosine Kinase Inhibitors via Tumor-Derived Exosome and T Cell Modulation.

Greenberg JW, Kim H, Ahn M, Moustafa AA, Zhou H, Barata PC, Boulares AH, Abdel-Mageed AB, Krane LS
Cancers 2022 Feb 11;14(4)

Low-metastatic melanoma cells acquire enhanced metastatic capability via exosomal transfer of miR-199a-1-5p from highly metastatic melanoma cells.

Zhao Q, Chen H, Li X, Zeng B, Sun Z, Liu D, Chen Y, Zhang Y, Rosie Xing H, Wang J
Cell death discovery 2022 Apr 9;8(1):188

Blockade of Exosome Release Suppresses Atrial Fibrillation by Alleviating Atrial Fibrosis in Canines With Prolonged Atrial Pacing.

Yao Y, He S, Wang Y, Cao Z, Liu D, Fu Y, Chen H, Wang X, Zhao Q
Frontiers in cardiovascular medicine 2021;8:699175

Exosomal CD47 Plays an Essential Role in Immune Evasion in Ovarian Cancer.

Shimizu A, Sawada K, Kobayashi M, Yamamoto M, Yagi T, Kinose Y, Kodama M, Hashimoto K, Kimura T
Molecular cancer research : MCR 2021 Sep;19(9):1583-1595

Morphological and histochemical characterization of the secretory epithelium in the canine lacrimal gland.

Yasui T, Miyata K, Nakatsuka C, Tsukise A, Gomi H
European journal of histochemistry : EJH 2021 Nov 2;65(4)

Repurposing ketoconazole as an exosome directed adjunct to sunitinib in treating renal cell carcinoma.

Greenberg JW, Kim H, Moustafa AA, Datta A, Barata PC, Boulares AH, Abdel-Mageed AB, Krane LS
Scientific reports 2021 May 13;11(1):10200

Particulate matter promotes hyperpigmentation via AhR/MAPK signaling activation and by increasing α-MSH paracrine levels in keratinocytes.

Shi Y, Zeng Z, Liu J, Pi Z, Zou P, Deng Q, Ma X, Qiao F, Xiong W, Zhou C, Zeng Q, Xiao R
Environmental pollution (Barking, Essex : 1987) 2021 Jun 1;278:116850

FOXO1-Mediated Downregulation of RAB27B Leads to Decreased Exosome Secretion in Diabetic Kidneys.

Zeng M, Wen J, Ma Z, Xiao L, Liu Y, Kwon S, Liu Y, Dong Z
Diabetes 2021 Jul;70(7):1536-1548

IKKβ activation promotes amphisome formation and extracellular vesicle secretion in tumor cells.

Peng X, Yang L, Ma Y, Li X, Yang S, Li Y, Wu B, Tang S, Zhang F, Zhang B, Liu J, Li H
Biochimica et biophysica acta. Molecular cell research 2021 Jan;1868(1):118857

Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer cachexia.

Hu W, Xiong H, Ru Z, Zhao Y, Zhou Y, Xie K, Xiao W, Xiong Z, Wang C, Yuan C, Shi J, Du Q, Zhang X, Yang H
Cell death & disease 2021 Jan 28;12(1):134

Rab27a Contributes to Cathepsin S Secretion in Lacrimal Gland Acinar Cells.

Fu R, Edman MC, Hamm-Alvarez SF
International journal of molecular sciences 2021 Feb 5;22(4)

RAB27A-dependent release of exosomes by liver cancer stem cells induces Nanog expression in their differentiated progenies and confers regorafenib resistance.

Huang H, Hou J, Liu K, Liu Q, Shen L, Liu B, Lu Q, Zhang N, Che L, Li J, Jiang S, Wang B, Wen Q, Hu L, Gao J
Journal of gastroenterology and hepatology 2021 Dec;36(12):3429-3437

Mouse 4T1 Breast Cancer Cell-Derived Exosomes Induce Proinflammatory Cytokine Production in Macrophages via miR-183.

Guo J, Duan Z, Zhang C, Wang W, He H, Liu Y, Wu P, Wang S, Song M, Chen H, Chen C, Si Q, Xiang R, Luo Y
Journal of immunology (Baltimore, Md. : 1950) 2020 Nov 15;205(10):2916-2925

DNA Damage Regulates Senescence-Associated Extracellular Vesicle Release via the Ceramide Pathway to Prevent Excessive Inflammatory Responses.

Hitomi K, Okada R, Loo TM, Miyata K, Nakamura AJ, Takahashi A
International journal of molecular sciences 2020 May 25;21(10)

Rab27a Contributes to the Processing of Inflammatory Pain in Mice.

Gross T, Wack G, Syhr KMJ, Tolmachova T, Seabra MC, Geisslinger G, Niederberger E, Schmidtko A, Kallenborn-Gerhardt W
Cells 2020 Jun 18;9(6)

Foot-and-mouth disease virus degrades Rab27a to suppress the exosome-mediated antiviral immune response.

Xu G, Xu S, Shi X, Shen C, Zhang D, Zhang T, Hou J, Zhang K, Zheng H, Liu X
Veterinary microbiology 2020 Dec;251:108889

Inhibition of the Notch1 Pathway Promotes the Effects of Nucleus Pulposus Cell-Derived Exosomes on the Differentiation of Mesenchymal Stem Cells into Nucleus Pulposus-Like Cells in Rats.

Lan WR, Pan S, Li HY, Sun C, Chang X, Lu K, Jiang CQ, Zuo R, Zhou Y, Li CQ
Stem cells international 2019;2019:8404168

Citrate reduced oxidative damage in stem cells by regulating cellular redox signaling pathways and represent a potential treatment for oxidative stress-induced diseases.

Wu X, Dai H, Liu L, Xu C, Yin Y, Yi J, Bielec MD, Han Y, Li S
Redox biology 2019 Feb;21:101057

Exosome Release Is Regulated by mTORC1.

Zou W, Lai M, Zhang Y, Zheng L, Xing Z, Li T, Zou Z, Song Q, Zhao X, Xia L, Yang J, Liu A, Zhang H, Cui ZK, Jiang Y, Bai X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2019 Feb 6;6(3):1801313

Microvesicle-Mediated Delivery of Minicircle DNA Results in Effective Gene-Directed Enzyme Prodrug Cancer Therapy.

Kanada M, Kim BD, Hardy JW, Ronald JA, Bachmann MH, Bernard MP, Perez GI, Zarea AA, Ge TJ, Withrow A, Ibrahim SA, Toomajian V, Gambhir SS, Paulmurugan R, Contag CH
Molecular cancer therapeutics 2019 Dec;18(12):2331-2342

Zoledronate modulates intracellular vesicle trafficking in mast cells via disturbing the interaction of myosinVa/Rab3a and sytaxin4/VAMP7.

Liu S, Sahid MNA, Takemasa E, Maeyama K, Mogi M
Biochemical pharmacology 2018 May;151:18-25

High-throughput screening identified selective inhibitors of exosome biogenesis and secretion: A drug repurposing strategy for advanced cancer.

Datta A, Kim H, McGee L, Johnson AE, Talwar S, Marugan J, Southall N, Hu X, Lal M, Mondal D, Ferrer M, Abdel-Mageed AB
Scientific reports 2018 May 25;8(1):8161

A RAB27A 5' untranslated region structural variant associated with late-onset hemophagocytic lymphohistiocytosis and normal pigmentation.

Tesi B, Rascon J, Chiang SCC, Burnyte B, Löfstedt A, Fasth A, Heizmann M, Juozapaite S, Kiudeliene R, Kvedaraite E, Miseviciene V, Muleviciene A, Müller ML, Nordenskjöld M, Matuzeviciene R, Samaitiene R, Speckmann C, Stankeviciene S, Zekas V, Voss M, Ehl S, Vaiciene-Magistris N, Henter JI, Meeths M, Bryceson YT
The Journal of allergy and clinical immunology 2018 Jul;142(1):317-321.e8

Essential Role of the a3 Isoform of V-ATPase in Secretory Lysosome Trafficking via Rab7 Recruitment.

Matsumoto N, Sekiya M, Tohyama K, Ishiyama-Matsuura E, Sun-Wada GH, Wada Y, Futai M, Nakanishi-Matsui M
Scientific reports 2018 Apr 30;8(1):6701

Exosome-mediated miR-146a transfer suppresses type I interferon response and facilitates EV71 infection.

Fu Y, Zhang L, Zhang F, Tang T, Zhou Q, Feng C, Jin Y, Wu Z
PLoS pathogens 2017 Sep;13(9):e1006611

Rab27A overexpression promotes bladder cancer proliferation and chemoresistance through regulation of NF-κB signaling.

Liu J, Gong X, Zhu X, Xue D, Liu Y, Wang P
Oncotarget 2017 Sep 26;8(43):75272-75283

Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties.

Gomi H, Osawa H, Uno R, Yasui T, Hosaka M, Torii S, Tsukise A
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2017 Nov;65(11):637-653

Manumycin A suppresses exosome biogenesis and secretion via targeted inhibition of Ras/Raf/ERK1/2 signaling and hnRNP H1 in castration-resistant prostate cancer cells.

Datta A, Kim H, Lal M, McGee L, Johnson A, Moustafa AA, Jones JC, Mondal D, Ferrer M, Abdel-Mageed AB
Cancer letters 2017 Nov 1;408:73-81

Autophagy is dispensable for Kmt2a/Mll-Mllt3/Af9 AML maintenance and anti-leukemic effect of chloroquine.

Chen X, Clark J, Wunderlich M, Fan C, Davis A, Chen S, Guan JL, Mulloy JC, Kumar A, Zheng Y
Autophagy 2017 May 4;13(5):955-966

Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.

Takahashi A, Okada R, Nagao K, Kawamata Y, Hanyu A, Yoshimoto S, Takasugi M, Watanabe S, Kanemaki MT, Obuse C, Hara E
Nature communications 2017 May 16;8:15287

Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-γ-stimulated lung epithelial cells.

Chen YD, Fang YT, Cheng YL, Lin CF, Hsu LJ, Wang SY, Anderson R, Chang CP, Lin YS
Scientific reports 2017 Jul 18;7(1):5676

Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation.

Wang HH, Cui Q, Zhang T, Wang ZB, Ouyang YC, Shen W, Ma JY, Schatten H, Sun QY
Histochemistry and cell biology 2016 Jun;145(6):647-57

Extracellular vesicles released by CD40/IL-4-stimulated CLL cells confer altered functional properties to CD4+ T cells.

Smallwood DT, Apollonio B, Willimott S, Lezina L, Alharthi A, Ambrose AR, De Rossi G, Ramsay AG, Wagner SD
Blood 2016 Jul 28;128(4):542-52

Expression of Secretogranin III in Chicken Endocrine Cells: Its Relevance to the Secretory Granule Properties of Peptide Prohormone Processing and Bioactive Amine Content.

Gomi H, Morikawa S, Shinmura N, Moki H, Yasui T, Tsukise A, Torii S, Watanabe T, Maeda Y, Hosaka M
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2015 May;63(5):350-66

Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion.

Asano S, Nemoto T, Kitayama T, Harada K, Zhang J, Harada K, Tanida I, Hirata M, Kanematsu T
Biology open 2014 May 8;3(6):463-74

Unexpected gain of function for the scaffolding protein plectin due to mislocalization in pancreatic cancer.

Shin SJ, Smith JA, Rezniczek GA, Pan S, Chen R, Brentnall TA, Wiche G, Kelly KA
Proceedings of the National Academy of Sciences of the United States of America 2013 Nov 26;110(48):19414-9

Golgi fragmentation is Rab and SNARE dependent in cellular models of Parkinson's disease.

Rendón WO, Martínez-Alonso E, Tomás M, Martínez-Martínez N, Martínez-Menárguez JA
Histochemistry and cell biology 2013 May;139(5):671-84

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Li J, Liu K, Liu Y, Xu Y, Zhang F, Yang H, Liu J, Pan T, Chen J, Wu M, Zhou X, Yuan Z
Nature immunology 2013 Aug;14(8):793-803

Cdx2 regulates endo-lysosomal function and epithelial cell polarity.

Gao N, Kaestner KH
Genes & development 2010 Jun 15;24(12):1295-305

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HL-60 cells were subjected to SDS PAGE followed by western blot with 17817-1-AP(RAB27A antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using RAB27A antibody 17817-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human liver cancer using 17817-1-AP(RAB27A antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human liver cancer using 17817-1-AP(RAB27A antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue