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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [6]
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- Product number
- NBP1-83327 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83327, RRID:AB_11006457
- Product name
- Rabbit Polyclonal Rab27a Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Rab27a antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rab27a Antibody [NBP1-83327] - Analysis in human cell lines SK-MEL-30 and Caco-2 using Anti-RAB27A antibody. Corresponding RAB27A RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327] - Staining of human stomach shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327] - Staining of human prostate shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327] - Staining of human kidney shows no cytoplasmic positivity in cells in tubules as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327] - Analysis in human stomach and skeletal muscle tissues. Corresponding RAB27A RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells with additional stron membranous positivity in a subset of glandular cells.
