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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AB0045-200 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB0045-200, RRID:AB_2333185
- Product name
- Anti-Rab27a
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody to Rab27b. Rab27b belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and involved in vesicle transport and small GTPase mediated signal transduction.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 400 µg
- Concentration
- 2 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Isolation of Intact and Functional Melanosomes from the Retinal Pigment Epithelium.
Rab27a GTPase modulates L-type Ca2+ channel function via interaction with the II-III linker of CaV1.3 subunit.
Pelkonen L, Reinisalo M, Morin-Picardat E, Kidron H, Urtti A
PloS one 2016;11(8):e0160352
PloS one 2016;11(8):e0160352
Rab27a GTPase modulates L-type Ca2+ channel function via interaction with the II-III linker of CaV1.3 subunit.
Reichhart N, Markowski M, Ishiyama S, Wagner A, Crespo-Garcia S, Schorb T, Ramalho JS, Milenkovic VM, Föckler R, Seabra MC, Strauß O
Cellular signalling 2015 Nov;27(11):2231-40
Cellular signalling 2015 Nov;27(11):2231-40
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cell lysates at 100 µg per lane
- Sample type
- AtT-20, Melan-Ink
- Primary Ab dilution
- 1/500
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- paraformaldehyde-fixed paraffin-embedded tissue
- Sample type
- mouse stomach
- Primary Ab dilution
- 1/200
- Conjugate
- Unconjugated
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval;
- Sample type
- mammary tissue
- Primary Ab dilution
- 1/200
