Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX129127 - Provider product page
- Provider
- GeneTex
- Product name
- DNMT3B antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
Submitted references Epigenetic targeting DNMT1 of pancreatic ductal adenocarcinoma using interstitial control release biodegrading polymer reduced tumor growth through hedgehog pathway inhibition.
The inhibitory activity of gallic acid against DNA methylation: application of gallic acid on epigenetic therapy of human cancers.
Procainamide Inhibits DNA Methylation and Alleviates Multiple Organ Dysfunction in Rats with Endotoxic Shock.
The role of DNA methylation during anoxia tolerance in a freshwater turtle (Trachemys scripta elegans).
Huang MH, Chou YW, Li MH, Shih TE, Lin SZ, Chuang HM, Chiou TW, Su HL, Harn HJ
Pharmacological research 2019 Jan;139:50-61
Pharmacological research 2019 Jan;139:50-61
The inhibitory activity of gallic acid against DNA methylation: application of gallic acid on epigenetic therapy of human cancers.
Weng YP, Hung PF, Ku WY, Chang CY, Wu BH, Wu MH, Yao JY, Yang JR, Lee CH
Oncotarget 2018 Jan 2;9(1):361-374
Oncotarget 2018 Jan 2;9(1):361-374
Procainamide Inhibits DNA Methylation and Alleviates Multiple Organ Dysfunction in Rats with Endotoxic Shock.
Shih CC, Liao MH, Hsiao TS, Hii HP, Shen CH, Chen SJ, Ka SM, Chang YL, Wu CC
PloS one 2016;11(9):e0163690
PloS one 2016;11(9):e0163690
The role of DNA methylation during anoxia tolerance in a freshwater turtle (Trachemys scripta elegans).
Wijenayake S, Storey KB
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 2016 Apr;186(3):333-42
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology 2016 Apr;186(3):333-42
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNMT3B antibody detects DNMT3B protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g whole cell lysate/extract of 3x-Flag human DNMT3B-transfected 293T cells5 % SDS-PAGEDNMT3B antibody (GTX129127) dilution: 1:5000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNMT3B antibody detects DNMT3B protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody (GTX129127) diluted by 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and DNMT3B knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody (GTX129127) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and DNMT3B knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody (GTX129127) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNMT3B antibody detects DNMT3B protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNMT3B protein stained by DNMT3B antibody (GTX129127) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of DNMT3B protein from K562 nuclear extracts using 5 £gg of DNMT3B antibody (GTX129127).Western blot analysis was performed using DNMT3B antibody (GTX129127).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNMT3B antibody detects DNMT3B protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. DNMT3B antibody (GTX129127) diluted at 1:250.