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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP1035a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP1035a, RRID:AB_2094134
- Product name
- Dnmt3b Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Functional switching of TGF-beta1 signaling in liver cancer via epigenetic modulation of a single CpG site in TTP promoter.
Systems-level dynamic analyses of fate change in murine embryonic stem cells.
DNA methyltransferase expression in the human endometrium: down-regulation by progesterone and estrogen.
MicroRNA-29 family reverts aberrant methylation in lung cancer by targeting DNA methyltransferases 3A and 3B.
Role of epigenetic effectors in maintenance of the long-term persistent bystander effect in spleen in vivo.
Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein.
Irradiation induces DNA damage and modulates epigenetic effectors in distant bystander tissue in vivo.
Effect of long-term tamoxifen exposure on genotoxic and epigenetic changes in rat liver: implications for tamoxifen-induced hepatocarcinogenesis.
Fractionated low-dose radiation exposure leads to accumulation of DNA damage and profound alterations in DNA and histone methylation in the murine thymus.
Epigenetic reactivation of tumor suppressor genes by a novel small-molecule inhibitor of human DNA methyltransferases.
Sohn BH, Park IY, Lee JJ, Yang SJ, Jang YJ, Park KC, Kim DJ, Lee DC, Sohn HA, Kim TW, Yoo HS, Choi JY, Bae YS, Yeom YI
Gastroenterology 2010 May;138(5):1898-908
Gastroenterology 2010 May;138(5):1898-908
Systems-level dynamic analyses of fate change in murine embryonic stem cells.
Lu R, Markowetz F, Unwin RD, Leek JT, Airoldi EM, MacArthur BD, Lachmann A, Rozov R, Ma'ayan A, Boyer LA, Troyanskaya OG, Whetton AD, Lemischka IR
Nature 2009 Nov 19;462(7271):358-62
Nature 2009 Nov 19;462(7271):358-62
DNA methyltransferase expression in the human endometrium: down-regulation by progesterone and estrogen.
Yamagata Y, Asada H, Tamura I, Lee L, Maekawa R, Taniguchi K, Taketani T, Matsuoka A, Tamura H, Sugino N
Human reproduction (Oxford, England) 2009 May;24(5):1126-32
Human reproduction (Oxford, England) 2009 May;24(5):1126-32
MicroRNA-29 family reverts aberrant methylation in lung cancer by targeting DNA methyltransferases 3A and 3B.
Fabbri M, Garzon R, Cimmino A, Liu Z, Zanesi N, Callegari E, Liu S, Alder H, Costinean S, Fernandez-Cymering C, Volinia S, Guler G, Morrison CD, Chan KK, Marcucci G, Calin GA, Huebner K, Croce CM
Proceedings of the National Academy of Sciences of the United States of America 2007 Oct 2;104(40):15805-10
Proceedings of the National Academy of Sciences of the United States of America 2007 Oct 2;104(40):15805-10
Role of epigenetic effectors in maintenance of the long-term persistent bystander effect in spleen in vivo.
Koturbash I, Boyko A, Rodriguez-Juarez R, McDonald RJ, Tryndyak VP, Kovalchuk I, Pogribny IP, Kovalchuk O
Carcinogenesis 2007 Aug;28(8):1831-8
Carcinogenesis 2007 Aug;28(8):1831-8
Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein.
Park IY, Sohn BH, Yu E, Suh DJ, Chung YH, Lee JH, Surzycki SJ, Lee YI
Gastroenterology 2007 Apr;132(4):1476-94
Gastroenterology 2007 Apr;132(4):1476-94
Irradiation induces DNA damage and modulates epigenetic effectors in distant bystander tissue in vivo.
Koturbash I, Rugo RE, Hendricks CA, Loree J, Thibault B, Kutanzi K, Pogribny I, Yanch JC, Engelward BP, Kovalchuk O
Oncogene 2006 Jul 20;25(31):4267-75
Oncogene 2006 Jul 20;25(31):4267-75
Effect of long-term tamoxifen exposure on genotoxic and epigenetic changes in rat liver: implications for tamoxifen-induced hepatocarcinogenesis.
Tryndyak VP, Muskhelishvili L, Kovalchuk O, Rodriguez-Juarez R, Montgomery B, Churchwell MI, Ross SA, Beland FA, Pogribny IP
Carcinogenesis 2006 Aug;27(8):1713-20
Carcinogenesis 2006 Aug;27(8):1713-20
Fractionated low-dose radiation exposure leads to accumulation of DNA damage and profound alterations in DNA and histone methylation in the murine thymus.
Pogribny I, Koturbash I, Tryndyak V, Hudson D, Stevenson SM, Sedelnikova O, Bonner W, Kovalchuk O
Molecular cancer research : MCR 2005 Oct;3(10):553-61
Molecular cancer research : MCR 2005 Oct;3(10):553-61
Epigenetic reactivation of tumor suppressor genes by a novel small-molecule inhibitor of human DNA methyltransferases.
Brueckner B, Garcia Boy R, Siedlecki P, Musch T, Kliem HC, Zielenkiewicz P, Suhai S, Wiessler M, Lyko F
Cancer research 2005 Jul 15;65(14):6305-11
Cancer research 2005 Jul 15;65(14):6305-11
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of anti-Dnmt3b Pab (Cat. #AP1035a) in T47-D cell lysate. Dnmt3b (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody (Cat.#AP1035a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
