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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
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- Product number
- MA1-23263 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NBS1 Monoclonal Antibody (1C3)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Recommended positive controls: 293T, MCF7, HeLa, Raji.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1C3
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Double strand breaks (DSBs) as indicators of genomic instability in PATRR-mediated translocations.
Correll-Tash S, Lilley B, Salmons Iv H, Mlynarski E, Franconi CP, McNamara M, Woodbury C, Easley CA, Emanuel BS
Human molecular genetics 2021 Feb 25;29(24):3872-3881
Human molecular genetics 2021 Feb 25;29(24):3872-3881
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NBS1 in Various whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with NBS1 Monoclonal antibody (Product # MA1-23263) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NBS1 in HeLa nuclear extract using a NBS1 monoclonal antibody (Product # MA1-23263).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NBS1 Monoclonal Antibody (1C3) (Product # MA1-23263). Sample (50 µg of whole cell lysate). Lane A: mouse brain . 7.5% SDS PAGE. NBS1 Monoclonal Antibody (1C3) (Product # MA1-23263) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NBS1 Monoclonal Antibody (1C3) (Product # MA1-23263). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 Monoclonal Antibody (1C3) (Product # MA1-23263) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NBS1 in HeLa cells using a NBS1 monoclonal antibody (Product # MA1-23263) at a 1:100 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa, using NBS1 (Product # MA1-23263) antibody at 1:100 dilution.
