MA1-23265

antibody from Invitrogen Antibodies

Targeting: NBN AT-V1, AT-V2, ATV, NBS, NBS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA1-23265

Provider

Invitrogen Antibodies

Product name

NBS1 Monoclonal Antibody (1D7)

Antibody type

Monoclonal

Antigen

Other

Description

Predicted molecular weight 91 kDa. Positive control: MCF7, HeLa (NE), Raji.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

1D7

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NBS1 in (A) HeLa and (B) HepG2 whole cell extract using a NBS1 monoclonal antibody (Product # MA1-23265).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NBS1 in Various whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with NBS1 Monoclonal antibody (Product # MA1-23265) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NBS1 Monoclonal Antibody (1D7) (Product # MA1-23265). Sample (30 µg of whole cell lysate). Lane A: HepG2. 7.5% SDS PAGE. NBS1 Monoclonal Antibody (1D7) (Product # MA1-23265) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NBS1 Monoclonal Antibody (1D7) (Product # MA1-23265). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 Monoclonal Antibody (1D7) (Product # MA1-23265) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockout of NBS1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR791917_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of NBS1 was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) and HeLa NBS1 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-NBS1 Monoclonal Antibody (1D7) (Product # MA1-23265, 1:1,000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to NBS1.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NBS1 in HeLa cells using a NBS1 monoclonal antibody (Product # MA1-23265) at a 1:100 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NBS1 Monoclonal Antibody (1D7) detects NBS1 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NBS1 stained by NBS1 Monoclonal Antibody (1D7) (Product # MA1-23265) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NBS1 Monoclonal Antibody (1D7) detects NBS1 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NBS1 stained by NBS1 Monoclonal Antibody (1D7) (Product # MA1-23265) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200.