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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX103229 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX103229, RRID:AB_2616070
- Product name
- NBS1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references PHRF1 promotes genome integrity by modulating non-homologous end-joining.
Chang CF, Chu PC, Wu PY, Yu MY, Lee JY, Tsai MD, Chang MS
Cell death & disease 2015 Apr 9;6:e1716
Cell death & disease 2015 Apr 9;6:e1716
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (GTX103229) diluted at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NBS1 antibody detects NBS1 protein by western blot analysis.A. 30 £gg NT2D1 whole cell extract7.5 % SDS-PAGENBS1 antibody (GTX103229) dilution: 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (GTX103229) diluted at 1:2000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody (GTX103229) diluted at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NBS1 antibody detects NBS1 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NBS1 protein stained by NBS1 antibody (GTX103229) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NBS1 antibody immunoprecipitates nibrin protein in IP experiments.IP samples: NT2D1 whole cell extractA. 50 £gg NT2D1 whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of nibrin protein by 4 £gg NBS1 antibody (GTX103229)7.5 % SDS-PAGEThe immunoprecipitated nibrin protein was detected by NBS1 antibody (GTX103229) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
