Explore
Validate
Learn
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB15731 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Nbs1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse Nbs1.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation of Human Nbs1. Nbs1 was immunoprecipitated from 500 μg of K562 human chronic myelogenous leukemia cell line lysate following incubation with 2 µg Mouse Anti-Human/Mouse Nbs1 Mono-clonal Antibody (Catalog # MAB15731) for 1 hour on ice. Nbs1-antibody complexes were absorbed using goat anti-mouse IgG-agarose (Sigma). Immunoprecipitated Nbs1 was detected by Western blot using 1 µg/mL Human/Mouse/Rat Nbs1 Mono-clonal Antibody (Catalog # MAB1573). View our recommended buffer recipes for immunoprecipitation.
