AF4944

antibody from Novus Biologicals

Targeting: NBN AT-V1, AT-V2, ATV, NBS, NBS1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4944

Provider

Novus Biologicals

Product name

Rabbit Polyclonal Nbs1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Nbs1 when phosphorylated at S343. Does not recognize Nbs1 when unphosphorylated at S343.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Phospho-Nbs1 (S343) by Western Blot. Western blot shows Nbs1 immunoprecipitated from lysates of HeLa human cervical epithelial carcinoma cell line using Human Nbs1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1573). HeLa cell line was mock treated or exposed to 100 J/m² UV-C for 3 hours. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-Nbs1 (S343) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4944) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Nbs1 (S343) was detected at approximately 95 kDa (as indicated). For additional reference the membrane was stripped and reprobed with 1 µg/mL Human/Mouse/Rat Nbs1 Monoclonal Antibody (lower panel, Catalog # MAB1573) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.