Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
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- Product number
- GTX70222 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX70222, RRID:AB_372443
- Product name
- NBS1 antibody [1C3]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
Submitted references Localization of Double-Strand Break Repair Proteins to Viral Replication Compartments following Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus.
The MRN complex is transcriptionally regulated by MYCN during neural cell proliferation to control replication stress.
The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers.
The telomere deprotection response is functionally distinct from the genomic DNA damage response.
CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors.
ATM-dependent cellular response to DNA double strand breaks plays a pivotal role in the maintenance of the integrity of the genome.
Physical interaction between the histone acetyl transferase Tip60 and the DNA double-strand breaks sensor MRN complex.
Importin KPNA2 is required for proper nuclear localization and multiple functions of NBS1.
Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability.
NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors.
Hollingworth R, Horniblow RD, Forrest C, Stewart GS, Grand RJ
Journal of virology 2017 Nov 15;91(22)
Journal of virology 2017 Nov 15;91(22)
The MRN complex is transcriptionally regulated by MYCN during neural cell proliferation to control replication stress.
Petroni M, Sardina F, Heil C, SahĂșn-Roncero M, Colicchia V, Veschi V, Albini S, Fruci D, Ricci B, Soriani A, Di Marcotullio L, Screpanti I, Gulino A, Giannini G
Cell death and differentiation 2016 Feb;23(2):197-206
Cell death and differentiation 2016 Feb;23(2):197-206
The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers.
Harten SK, Oey H, Bourke LM, Bharti V, Isbel L, Daxinger L, Faou P, Robertson N, Matthews JM, Whitelaw E
BMC biology 2015 Mar 26;13:21
BMC biology 2015 Mar 26;13:21
The telomere deprotection response is functionally distinct from the genomic DNA damage response.
Cesare AJ, Hayashi MT, Crabbe L, Karlseder J
Molecular cell 2013 Jul 25;51(2):141-55
Molecular cell 2013 Jul 25;51(2):141-55
CCT241533 is a potent and selective inhibitor of CHK2 that potentiates the cytotoxicity of PARP inhibitors.
Anderson VE, Walton MI, Eve PD, Boxall KJ, Antoni L, Caldwell JJ, Aherne W, Pearl LH, Oliver AW, Collins I, Garrett MD
Cancer research 2011 Jan 15;71(2):463-72
Cancer research 2011 Jan 15;71(2):463-72
ATM-dependent cellular response to DNA double strand breaks plays a pivotal role in the maintenance of the integrity of the genome.
Suzuki K, Yamauchi M, Yamashita S
Radiation protection dosimetry 2011 Feb;143(2-4):279-83
Radiation protection dosimetry 2011 Feb;143(2-4):279-83
Physical interaction between the histone acetyl transferase Tip60 and the DNA double-strand breaks sensor MRN complex.
Chailleux C, Tyteca S, Papin C, Boudsocq F, Puget N, Courilleau C, Grigoriev M, Canitrot Y, Trouche D
The Biochemical journal 2010 Feb 24;426(3):365-71
The Biochemical journal 2010 Feb 24;426(3):365-71
Importin KPNA2 is required for proper nuclear localization and multiple functions of NBS1.
Tseng SF, Chang CY, Wu KJ, Teng SC
The Journal of biological chemistry 2005 Nov 25;280(47):39594-600
The Journal of biological chemistry 2005 Nov 25;280(47):39594-600
Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability.
Giannini G, Rinaldi C, Ristori E, Ambrosini MI, Cerignoli F, Viel A, Bidoli E, Berni S, D'Amati G, Scambia G, Frati L, Screpanti I, Gulino A
Oncogene 2004 Apr 8;23(15):2640-7
Oncogene 2004 Apr 8;23(15):2640-7
NFBD1/MDC1 regulates ionizing radiation-induced focus formation by DNA checkpoint signaling and repair factors.
Xu X, Stern DF
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2003 Oct;17(13):1842-8
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2003 Oct;17(13):1842-8
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1C3] (GTX70222) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Detection of human NBS1 protein in HeLa nuclear extract using GeneTex NBS1 1C3 monoclonal antibody GTX70222.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX70222 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1C3] (GTX70222) diluted at1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1C3] (GTX70222) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa, using NBS1(GTX70222) antibody at 1:100 dilution.