UM800029

antibody from Invitrogen Antibodies

Targeting: NBN AT-V1, AT-V2, ATV, NBS, NBS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

UM800029

Provider

Invitrogen Antibodies

Product name

NBN Monoclonal Antibody (UMAB99), UltraMAB™

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

UMAB99

Vial size

100 µL

Concentration

0.5-1.0 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of human tissue lysates (15 µg) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Dilution: 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-NBN monoclonal antibody (Clone UMAB99).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and NBN-Knockout HeLa cells (KO, Cat# LC831313) were separated by SDS-PAGE and immunoblotted with anti-NBN monoclonal antibody UM800029(1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of A549 cells using NBN mouse monoclonal antibody (UM800029, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-NBN mouse monoclonal antibody (UM800029, green, 1:100). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human prostate tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human lymphoma tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NBN mouse monoclonal antibody. (UM800029; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NBN mouse monoclonal antibody (UUM800029). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.