PA3-16528

antibody from Invitrogen Antibodies

Targeting: NBN AT-V1, AT-V2, ATV, NBS, NBS1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA3-16528

Provider

Invitrogen Antibodies

Product name

NBS1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

ICC/IF has been performed using methanol-fixed IMR90 primary human fibroblasts.

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NBS1 in human cell lysate using a polyclonal antibody (Product # PA3-16528).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockout validation by Western blot analysis of NBS1 in lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. Samples were incubated in NBS1 polyclonal antibody (Product # PA3-16528) using a dilution of 1:1000 followed by a HRP-conjugated Anti-Rabbit IgG secondary antibody. Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NBS1 in HeLa whole cell lysate. Samples were incubated in NBS1 polyclonal antibody (Product # PA3-16528). Observed Molecular Weight at ~95 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ICC staining of Hela cells using Product # PA3-16528 anti-NBS1.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of NBS1 in formalin-fixed paraffin-embedded human testis. Samples were incubated in NBS1 polyclonal antibody (Product # PA3-16528) using a dilution of 1:200. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.