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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- NB100-277 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-277, RRID:AB_10003114
- Product name
- Rabbit Polyclonal Nbs1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Cdc7-Dbf4 and the human S checkpoint response to UVC.
A role for MRE11, NBS1, and recombination junctions in replication and stable maintenance of EBV episomes.
Lack of Bcl11b tumor suppressor results in vulnerability to DNA replication stress and damages.
Heffernan TP, Unsal-Kaçmaz K, Heinloth AN, Simpson DA, Paules RS, Sancar A, Cordeiro-Stone M, Kaufmann WK
The Journal of biological chemistry 2007 Mar 30;282(13):9458-68
The Journal of biological chemistry 2007 Mar 30;282(13):9458-68
A role for MRE11, NBS1, and recombination junctions in replication and stable maintenance of EBV episomes.
Dheekollu J, Deng Z, Wiedmer A, Weitzman MD, Lieberman PM
PloS one 2007 Dec 5;2(12):e1257
PloS one 2007 Dec 5;2(12):e1257
Lack of Bcl11b tumor suppressor results in vulnerability to DNA replication stress and damages.
Kamimura K, Mishima Y, Obata M, Endo T, Aoyagi Y, Kominami R
Oncogene 2007 Aug 30;26(40):5840-50
Oncogene 2007 Aug 30;26(40):5840-50
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Nbs1 Antibody [NB100-277] - NBS1 Antibody [NB100-277] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NBS1 antibody (Lot #2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NBS1 was also immunoprecipitated by a prior batch (Lot #1) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
