Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF4944 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-Nbs1 (S343) Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Nbs1 when phosphorylated at S343. Does not recognize Nbs1 when unphosphorylated at S343.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Phospho-Nbs1 (S343) by Western Blot. Western blot shows Nbs1 immunoprecipitated from lysates of HeLa human cervical epithelial carcinoma cell line using Human Nbs1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1573). HeLa cell line was mock treated or exposed to 100 J/m2 UV-C for 3 hours. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-Nbs1 (S343) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4944) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Nbs1 (S343) was detected at approximately 95 kDa (as indicated). For additional reference the membrane was stripped and reprobed with 1 µg/mL Human/Mouse/Rat Nbs1 Monoclonal Antibody (lower panel, Catalog # MAB1573) This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
