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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP11931c - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP11931c, RRID:AB_10821820
- Product name
- C1QC Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- C1QC Antibody (Center) (Cat. #AP11931c) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the C1QC antibody detected the C1QC protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of C1QC Antibody (Center)(Cat#AP11931c) with WiDr cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- C1QC Antibody (Center) (Cat. #AP11931c)immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of C1QC Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- C1QC Antibody (Center) (Cat. #AP11931c) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
