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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-35084 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C1QC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C1QC in HL-60 cell line lysates. Samples were incubated with C1QC polyclonal antibody (Product # PA5-35084). Lysates: 35 µg/lane. C1QC protein (arrow).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of C1Q C in WiDr cells using a C1Q C polyclonal antibody (Product # PA5-35084) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of C1QC in WiDr cells. Samples were incubated in C1QC polyclonal antibody (Product # PA5-35084) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of C1QC in formalin fixed and paraffin embedded human rectum tissue. Samples were incubated with C1QC polyclonal antibody (Product # PA5-35084) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of C1Q C in HL-60 cells (right) compared to a negative control (left) using a C1Q C polyclonal antibody (Product # PA5-35084) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of C1QC in HL-60 cells (right histogram). Samples were incubated with C1QC polyclonal antibody (Product # PA5-35084) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).
