PAB10206

antibody from Abnova Corporation

Targeting: FBXO43 Fbx43

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10206

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10206, RRID:AB_1674686

Product name

FBXO43 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of FBXO43.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Systematic analysis and nomenclature of mammalian F-box proteins.

Jin J, Cardozo T, Lovering RC, Elledge SJ, Pagano M, Harper JW
Genes & development 2004 Nov 1;18(21):2573-80

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using FBXO43 polyclonal antibody (Cat # PAB10206).Panels A and B show two identical membranes containing marker, in vitro translated FBXO43 (FBXO43 ivt), and a whole cell extract from 293T cells loaded in duplicate.Panel A shows antibody reactivity.Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide.This pre-incubation greatly diminishes specific band recognition.Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells.When this experiment was repeated using a mock in vitro translation, no band was detected (data not shown).Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute.