Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB10206 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10206, RRID:AB_1674686
- Product name
- FBXO43 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of FBXO43.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Systematic analysis and nomenclature of mammalian F-box proteins.
Jin J, Cardozo T, Lovering RC, Elledge SJ, Pagano M, Harper JW
Genes & development 2004 Nov 1;18(21):2573-80
Genes & development 2004 Nov 1;18(21):2573-80
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using FBXO43 polyclonal antibody (Cat # PAB10206).Panels A and B show two identical membranes containing marker, in vitro translated FBXO43 (FBXO43 ivt), and a whole cell extract from 293T cells loaded in duplicate.Panel A shows antibody reactivity.Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide.This pre-incubation greatly diminishes specific band recognition.Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells.When this experiment was repeated using a mock in vitro translation, no band was detected (data not shown).Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute.