AP09239PU-N

antibody from Acris Antibodies GmbH

Targeting: FBXO43 Fbx43

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AP09239PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09239PU-N, RRID:AB_2035470

Product name

anti FBXO43

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to a region approximately 60 residues downstream of the amino terminal end of human Fbp5B protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.3 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using affinity purified anti-Fbp5B antibody. Panels A and B show two identical membranes containing marker, in vitro translated Fbp5b (Fbp5b ivt), and a whole cell extract from 293T cells loaded in duplicate). Panel A shows antibody reactivity. Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide. This pre-incubation greatly diminishes specific band recognition. Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells. When this experiment was repeated using a mock in vitro translation, no band was detected (data not shown). Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute