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- Antigen structure
- References [13]
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- Immunohistochemistry [2]
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- Product number
- AF2655 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat Complement Component C3d Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Complement Component C3d in direct ELISAs and Western blots. Detects mouse and rat Complement Component C3d in immunohistochemistry.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P01027- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Identifying the Role of Complement in Triggering Neuroinflammation after Traumatic Brain Injury.
Acute Complement Inhibition Potentiates Neurorehabilitation and Enhances tPA-Mediated Neuroprotection.
Complement Inhibitor CRIg/FH Ameliorates Renal Ischemia Reperfusion Injury via Activation of PI3K/AKT Signaling.
C3d regulates immune checkpoint blockade and enhances antitumor immunity.
Iron-induced Local Complement Component 3 (C3) Up-regulation via Non-canonical Transforming Growth Factor (TGF)-β Signaling in the Retinal Pigment Epithelium.
The epidermal growth factor receptor is a regulator of epidermal complement component expression and complement activation.
Soluble CR1 therapy improves complement regulation in C3 glomerulopathy.
Donor brain death exacerbates complement-dependent ischemia/reperfusion injury in transplanted hearts.
Targeting complement component 5a promotes vascular integrity and limits airway remodeling.
The alternative complement pathway propagates inflammation and injury in murine ischemic stroke.
Pathogenic natural antibodies propagate cerebral injury following ischemic stroke in mice.
Age-related retinopathy in NRF2-deficient mice.
Decay-accelerating factor suppresses complement C3 activation and retards atherosclerosis in low-density lipoprotein receptor-deficient mice.
Alawieh A, Langley EF, Weber S, Adkins D, Tomlinson S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Mar 7;38(10):2519-2532
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Mar 7;38(10):2519-2532
Acute Complement Inhibition Potentiates Neurorehabilitation and Enhances tPA-Mediated Neuroprotection.
Alawieh A, Andersen M, Adkins DL, Tomlinson S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Jul 18;38(29):6527-6545
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Jul 18;38(29):6527-6545
Complement Inhibitor CRIg/FH Ameliorates Renal Ischemia Reperfusion Injury via Activation of PI3K/AKT Signaling.
Hu C, Li L, Ding P, Li L, Ge X, Zheng L, Wang X, Wang J, Zhang W, Wang N, Gu H, Zhong F, Xu M, Rong R, Zhu T, Hu W
Journal of immunology (Baltimore, Md. : 1950) 2018 Dec 15;201(12):3717-3730
Journal of immunology (Baltimore, Md. : 1950) 2018 Dec 15;201(12):3717-3730
C3d regulates immune checkpoint blockade and enhances antitumor immunity.
Platt JL, Silva I, Balin SJ, Lefferts AR, Farkash E, Ross TM, Carroll MC, Cascalho M
JCI insight 2017 May 4;2(9)
JCI insight 2017 May 4;2(9)
Iron-induced Local Complement Component 3 (C3) Up-regulation via Non-canonical Transforming Growth Factor (TGF)-β Signaling in the Retinal Pigment Epithelium.
Li Y, Song D, Song Y, Zhao L, Wolkow N, Tobias JW, Song W, Dunaief JL
The Journal of biological chemistry 2015 May 8;290(19):11918-34
The Journal of biological chemistry 2015 May 8;290(19):11918-34
The epidermal growth factor receptor is a regulator of epidermal complement component expression and complement activation.
Abu-Humaidan AH, Ananthoju N, Mohanty T, Sonesson A, Alberius P, Schmidtchen A, Garred P, Sørensen OE
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3355-64
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3355-64
Soluble CR1 therapy improves complement regulation in C3 glomerulopathy.
Zhang Y, Nester CM, Holanda DG, Marsh HC, Hammond RA, Thomas LJ, Meyer NC, Hunsicker LG, Sethi S, Smith RJ
Journal of the American Society of Nephrology : JASN 2013 Nov;24(11):1820-9
Journal of the American Society of Nephrology : JASN 2013 Nov;24(11):1820-9
Donor brain death exacerbates complement-dependent ischemia/reperfusion injury in transplanted hearts.
Atkinson C, Floerchinger B, Qiao F, Casey S, Williamson T, Moseley E, Stoica S, Goddard M, Ge X, Tullius SG, Tomlinson S
Circulation 2013 Mar 26;127(12):1290-9
Circulation 2013 Mar 26;127(12):1290-9
Targeting complement component 5a promotes vascular integrity and limits airway remodeling.
Khan MA, Maasch C, Vater A, Klussmann S, Morser J, Leung LL, Atkinson C, Tomlinson S, Heeger PS, Nicolls MR
Proceedings of the National Academy of Sciences of the United States of America 2013 Apr 9;110(15):6061-6
Proceedings of the National Academy of Sciences of the United States of America 2013 Apr 9;110(15):6061-6
The alternative complement pathway propagates inflammation and injury in murine ischemic stroke.
Elvington A, Atkinson C, Zhu H, Yu J, Takahashi K, Stahl GL, Kindy MS, Tomlinson S
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 1;189(9):4640-7
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 1;189(9):4640-7
Pathogenic natural antibodies propagate cerebral injury following ischemic stroke in mice.
Elvington A, Atkinson C, Kulik L, Zhu H, Yu J, Kindy MS, Holers VM, Tomlinson S
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 1;188(3):1460-8
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 1;188(3):1460-8
Age-related retinopathy in NRF2-deficient mice.
Zhao Z, Chen Y, Wang J, Sternberg P, Freeman ML, Grossniklaus HE, Cai J
PloS one 2011 Apr 29;6(4):e19456
PloS one 2011 Apr 29;6(4):e19456
Decay-accelerating factor suppresses complement C3 activation and retards atherosclerosis in low-density lipoprotein receptor-deficient mice.
Leung VW, Yun S, Botto M, Mason JC, Malik TH, Song W, Paixao-Cavalcante D, Pickering MC, Boyle JJ, Haskard DO
The American journal of pathology 2009 Oct;175(4):1757-67
The American journal of pathology 2009 Oct;175(4):1757-67
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Complement Component C3d in Mouse Kidney. Complement Component C3d was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Complement Component C3d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2655) overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to basement membrane. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Complement Component C3d in Rat Kidney. Complement Component C3d was detected in perfusion fixed paraffin-embedded sections of rat kidney using Goat Anti-Mouse Complement Component C3d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2655) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
