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LearnCF501350
antibody from Invitrogen Antibodies
Targeting: CFAP298
C21orf48, C21orf59, CILD26, FBB18, FLJ20467, Kur
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- CF501350 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C21orf59 Monoclonal Antibody (OTI2H5), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI2H5
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C21orf59 (RC200169, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-C21. Positive lysates LY402864 (100 µg) and LC402864 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C21orf59 (RC200169, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-C21. Positive lysates LY402864 (100 µg) and LC402864 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10 µg) from 6 different cell lines by using anti-C21orf59 monoclonal antibody. (1:200)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-C21orf59 mouse monoclonal antibody (TA501350) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C21orf59(RC200169).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200169 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C21orf59 antibody (TA501350), and then analyzed by flow cytometry.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-C21orf59 antibody (TA501350), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
