LS-C375972

antibody from LSBio

Targeting: IRAK4 NY-REN-64

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C375972

Provider

LSBio

Product name

IRAK4 / IRAK-4 Antibody (aa1-220) LS-C375972

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating IRAK4 in MCF-7 whole cell lysate Lane 1: Rabbit control IgG instead of Rabbit anti-human Interleukin-1 receptor-associated kinase 4 polyclonal Antibody(IRAK4 ) in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Rabbit anti-human Interleukin-1 receptor-associated kinase 4 polyclonal Antibody(IRAK4 ) (8µg) + MCF-7 whole cell lysate (500µg) Lane 3: MCF-7 whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating IRAK4 in MCF-7 whole cell lysate Lane 1: Rabbit control IgG instead of Rabbit anti-human Interleukin-1 receptor-associated kinase 4 polyclonal Antibody(IRAK4 ) in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Rabbit anti-human Interleukin-1 receptor-associated kinase 4 polyclonal Antibody(IRAK4 ) (8µg) + MCF-7 whole cell lysate (500µg) Lane 3: MCF-7 whole cell lysate (10µg)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating IRAK4 in MCF-7 whole cell lysate Lane 1: Rabbit control IgG instead of Rabbit anti-human Interleukin-1 receptor-associated kinase 4 polyclonal Antibody(IRAK4 ) in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Rabbit anti-human Interleukin-1 receptor-associated kinase 4 polyclonal Antibody(IRAK4 ) (8µg) + MCF-7 whole cell lysate (500µg) Lane 3: MCF-7 whole cell lysate (10µg)